Effect of interferon-alpha-containing drugs on innate immunity reactions and population and subpopulation composition of peripheral blood lymphocytes

Introduction . Interferon-containing drugs are frequently used, but their effect on the innate immune cells response and adaptive immunity parameters is not well known. Aim To investigate of interferon-alpha-containing production neutrophil extracellular traps, population subpopulation composition peripheral blood lymphocytes in cell culture conditions. Materials methods We used from 12 healthy donors 6 patients with acute inflammation, which was as a lymphocyte source. Neutrophil traps were induced by two methods: either spontaneously, without stimulation, or lipopolysaccharides. Results discussions The investigation responses showed that neutrophils demonstrated slight spontaneous during incubation (4 h). significant NET increase observed after stimulation lipopolysaccharides up to 31.59 ± 2.32% 2-hour period, 42.93 3.56% 4-hour period. Viferon does have number neutrophilic significantly increases size. Kipferon limits excessive reducing these structures, also reduces size changes morphology. Ergoferon causes only rapid extremely long DNA fibers go beyond scope view when exposed Ergoferon. stimulates several morphological forms at once. Conclusion development mainly maintained Kipferon. At same time, restrains intensity inflammatory reaction active NK cells. conducted multilevel study allow researchers identify new properties mechanisms action administered pharmacological products containing interferon-α, confirm effective individual components system due action.